School of Medicine

Wayne State University School of Medicine

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Title
Distinguished Professor Emeritus
Distinguished Professor -Florida International University Herbert Wertheim College of Medicine
Phone
305-348-0657
Profile

For nearly four decades the Rosen laboratory has investigated the mechanisms of transport and detoxification of transition metals, heavy metals and metalloids in bacteria, yeast, protozoans, mammals and plants, from E. coli to humans. We identified most known arsenic detoxification genes and characterized their gene products at the biochemical and structural level.  In last few years we identified a new biogeocycle for organoarsenicals, including the ArsM As(III) SAM methyltransferase, the ArsI C-As lyase, the ArsH methylarsenite oxidase and the ArsP methylarsenite efflux permease. These enzymes and transporters transform and detoxify organoarsenical herbicides and antimicrobial growth promoters. Our research program is funded by NIH grants from NIGMS and NIEHS.

Graduate
Ph.D. University of Connecticut, 1969
Interests

Everything arsenic

Publications
  • Zhu, Y.G., Yoshinaga, M., Zhao, F. and Rosen, B.P. Earth abides arsenic biotransformations, Annu. Rev. Earth Planet. Sci. 42, 443–467 (2014). (Invited review)
  • Chen, J., Sun, D., Li, C.Z., Zhu, Y.G. and Rosen, B.P. A biosensor for trivalent organoarsenical herbicides and growth promoters, Environ Sci Technol. 48, 1141-1147 (2014)
  • Ye, J., Nadar, S.V., Li, J. and Rosen, B.P. Structure of Escherichia coli Grx2 in complex with glutathione: a dual-function hybrid of glutaredoxin and glutathione S-transferase Acta Cryst. D, 70, 761-614 (2014).
  • Yoshinaga, M. and Rosen, B.P. A C-As lyase for degradation of environmental organoarsenical herbicides and animal husbandry growth promoters, Proc. Natl. Acad. Sci. U.S.A. 111, 7701-7706 (2014).
  • Chen J, Sun, G.X., Wang, X.X., de Lorenzo, V., Rosen, B.P. and Zhu, Y.G. Volatilization of arsenic from polluted soil by Pseudomonas putida engineered for expression of the arsM arsenic(III) S-adenosine methyltransferase gene. Environ Sci Technol. 48, 10337-10344 (2014).
  • Pillai, J.K., Venkadesh, S., Abdul Ajees, A., Rosen, B.P. and Bhattacharjee, H. Mutations in the ArsA ATPase that restore interaction with the ArsD metallochaperone, Biometals 27, 1263-1275 (2014)
  • Dheeman, D.S., Packianathan, C., Pillai, J.K. and Rosen, B.P. The pathway of human AS3MT arsenic methylation. Chem Research in Toxicol., 27, 1979-1989 (2014).
  • Dong, H., Xu, W., Pillai, J.D., Packianathan, C. and Rosen, B.P. High-throughput screening-compatible assays of As(III) S-adenosylmethionine methyltransferase activity. Anal. Biochem. 480:67-73 (2015).
  • Marapakala, K., Packianathan, C., Abdul Ajees, A.A., Dheeman, D.S., Sankaran, B., Kandavelu, P. and Rosen, B.P. A disulfide bond cascade mechanism for As(III) S-adenosylmethionine methyltransferases. Acta Cryst. D. 71, 505-515 (2015).
  • Chen, J., Bhattacharjee, H. and Rosen, B.P. ArsH is an organoarsenical oxidase that confers resistance to trivalent forms of the herbicide MSMA and the poultry growth promoter roxarsone. Molec. Microbiol. 96, 1042-1052(2015).
  • Chen, J., Madegowda, M., Bhattacharjee, H. and Rosen, B.P., ArsP: a methylarsenite efflux permease. Molec. Microbiol. 98, 625–635 (2015).
  • Dong, H., Madegowda. M., Nefzi, A., Houghten, R.A., Giulianotti, M.A. and Rosen, B.P. Identification of small molecule inhibitors of human As(III) S-adenosylmethionine methyltransferase (AS3MT). Chem. Res. Toxicol. 21, 2419-2425 (2015).
  • Kumar, N.V., Yang. J., Pillai, J.K., Rawat, S., Solano, C. Kumar, A., Grøtli, M., Stemmler, T.L., Rosen, B.P., Tamás, M.J. Arsenic directly binds to and activates the yeast AP-1-like transcription factor Yap8, Molec. Cell. Biol. 36, 913-922 (2016).
  • Nadar, V.S., Yoshinaga, M., Pawitwar, S., Kandavelu, P., Sankaran, B. and Rosen, B.P. Structure of the ArsI C-As lyase: insights into the mechanism of degradation of organoarsenical herbicides and growth promoters. J. Molec. Biol. 428, 2462–2473 (2016).
  • Chen, J., Yoshinaga, M., Garbinski, L. and Rosen, B.P. Synergistic interaction of glyceraldehydes-3-phosphate dehydrogenase and ArsJ, a novel organoarsenical efflux permease, confers arsenate resistance. Molec. Microbiol. 100,945-953 (2016).
  • Li, J., Mandal, G. and Rosen, B.P. Expression of arsenic resistance genes in the obligate anaerobe Bacteroides vulgatus ATCC 8482, a gut microbiome bacterium. Anaerobes, 39,117-123(2016).
  • Chen, J. and Rosen, B.P. Organoarsenical biotransformations by Shewanella putrefaciens. Environ Sci Technol. 50,7956-7963(2016).
  • Duan, G. Hu, Y., McDermott, J., Chen, J., Schneider, S., Saur, N. Rosen, B.P., Liu, Z. and Zhu, Y.G. Inositol permeases AtINT2 and AtINT4 transport arsenic into Arabidopsis thaliana seeds. Nature Plants, 21,15202(2016).
  • Li, J., Pawitwar, S. and Rosen, B.P. The organoarsenical biocycle and the primordial antibiotic methylarsenite. Metallomics, 8,1047-1055(2016). (Invited review)